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Pyrene phosphoramidite dU

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  • 产品名称:Pyrene phosphoramidite dU
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  • 产品展商:Lumiprobe
  • 产品价格:0.00 元
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简单介绍

Pyrene phosphoramidite dU

产品描述

 

订购货号 产品名称及规格 价格(¥)
11460 Pyrene phosphoramidite dU, 100 mg 1950.00
21460 Pyrene phosphoramidite dU, 250 mg 4160.00
41460 Pyrene phosphoramidite dU, 1 g 11440.00

 


Pyrene is polycyclic aromatic hydrocarbon which is well-known for its ability to intercalate into DNA. Pyrene features intense blue fluorescence. Its parameters depend strongly on fluorophore microenvironment. Therefore, fluorescence spectra of pyrene are used for the extraction of structural information about site surrounding pyrene. Two pyrenes in close proximity usually form excimers easily detectable by excimer fluorescence. Pyrene can also be a FRET donor to other fluorophores such as perylene.
With this phosphoramidite, pyrene can be introduced into DNA or RNA by means of automated synthesis. This pyrene phosphoramidite contains hydrocarbon moiety rigidly attached to deoxyuridine. This reagent allows to attach pyrene fragment to 5', internal, or 3' position (using universal support).
This amidite requires no special handling, coupling, or deprotection conditions. Recommended diluent for it is acetonitrile.
 


General properties

 

Appearance:

yellowish foam

Molecular weight:

955.04

Molecular formula:

C57H55N4O8P

Solubility:

good in dichloromethane and acetonitrile

Quality control:

NMR 1H (95%) and 13C, TLC, HPLC

Storage conditions:

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

MSDS:

Download

Spectral properties

 

Excitation maximum, nm:

260; 282; 365; 392

Extinction coefficient at excitation maximum, Lmol-1cm-1:

12600; 21900; 16000; 14200

Emission maximum, nm:

460

Fluorescence quantum yield:

~0.1

 

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